| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sparaco, Isabella M (10)
|
MAEMAC |
41.24 |
39.95 |
-1.29 |
| 1 |
Anton, Lauren A (9)
|
MAJW |
|
50.31 |
- |
| 2 |
Suleski, Kyra G (10)
|
MAJW |
42.42 |
42.02 |
-0.40 |
| 2 |
Vinod, Aaditi (10)
|
MAJW |
50.18 |
50.37 |
0.19 |
| 3 |
Fisher, Paige E (10)
|
MAGRA |
41.75 |
42.54 |
0.79 |
| 3 |
Wagner, Kaitlyn F (10)
|
MAJW |
52.49 |
52.45 |
-0.04 |
| 4 |
Dalumpines, Angel Noelle M (10)
|
MAJW |
42.62 |
43.78 |
1.16 |
| 4 |
Li, Erin X (9)
|
MAJW |
52.84 |
53.40 |
0.56 |
| 5 |
Johnson, Karla A (9)
|
MAPRSC |
44.87 |
44.19 |
-0.68 |
| 5 |
Branscom, Raya (9)
|
MAJW |
55.86 |
53.93 |
-1.93 |
| 6 |
Poblador, Lianne L (10)
|
MAJW |
44.48 |
45.77 |
1.29 |
| 6 |
Tonghini, Isabella D (10)
|
MAJW |
|
56.78 |
- |
| 7 |
Cygan, Claire A (9)
|
MAPRSC |
1:06.72 |
1:04.74 |
-1.98 |
| 7 |
Barbich, Eva M (9)
|
MAPRSC |
46.45 |
47.44 |
0.99 |
| 8 |
Doppler, Olivia G (10)
|
MAPRSC |
44.72 |
47.53 |
2.81 |
| 9 |
Walsh, Kelly A (10)
|
MAWAVE |
47.28 |
47.75 |
0.47 |
| 10 |
Longo, Mia A (10)
|
MAJW |
46.54 |
48.42 |
1.88 |
|
Schwager, Breanna J (9)
|
MAPRSC |
1:05.26 |
DQ |
- |
|
Righetti, Sophia L (9)
|
MATVAC |
1:00.51 |
DQ |
- |
|
Kouser, Kaitlyn M (9)
|
MAJW |
55.37 |
NS |
- |
|
Lee, Katherine Y (9)
|
MAJW |
|
NS |
- |